H.PYLORI Anti Body Test

The H.pylori Antibody Test is a qualitative membrane based immunoassay for the detection of H.pylori antibodies in whole blood, serum or plasma. In this test procedure, anti-human IgG is immobilizied in the test line region of the test. After specimen is added to the specimen well of the device, it reacts with H.pylori antigen coated particles in the test. This mixture migrates chromatographically along the length of the test and interacts with the immobilized anti-human IgG. If the specimen contains H.pylori antibodies, a colored line will appear in the test line region indicating a positive result. If the specimen does not contain H.pylori antibodies, a colored line will not appear in this region indicating a negative result. To serve as a procedural control, a colored line will always appear in the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.

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Test Procdure

1. Bring the pouched test device to room temperature(15-30â„ƒ) prior to testing. Do not open the pouch until ready

to begin testing.

2. Remove the device from the sealed pouch and lay it on a flat and dry surface.

3. Using the provided pipette, add one drop of fresh specimen to the sample well.

4. Hold the buffer bottle vertically and add 1 drop to the sample well. /If using a pipette, change a new one to avoid

cross-contamination. Draw and transfer 2-3 drops of buffer to the sample well.

5. Read the result between 15-20minutes. Do not read results after 20 minutes.

H.PYLORI Anti Body Test

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